What are some possible sour..

what are some possible sources of error when doing a CHROMATOGRAPHY lab on SPINACH & RED CABBAGE pigments (chlorophyll a, chlorophyll b, anthocyanins...)

highschool lab

It`s a pitty, that you don`t say what sort of chromatography you are going to use, therefore here a site explaining them all:

http://www.itsjustabox.com/Sciencefair.h...

If you work precisely, take care that the spot is as small as possible, all spots have very similar concentration ( 5 drops each f.e. ), the level of solvent is not higher than the nethermost border of spots but not lower too, if you don`t move the chamber in the time of analysis no error should happen.

You will get precisely isolated pigments.

Here you find an example thin layer chromatography

( teacher note at the end:

http://209.85.135.104/search?q=cache:L-D...

and here paper chromatography:

http://media.nasaexplores.com/lessons/01...